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丙烯葡聚糖凝胶S-200 HR
来自 : 发布时间:2024-05-17
货号: 17-0584-10
CAS号: 65546-95-4
数量: 现货
英文名: Sephacryl S-200 High resolution
保存条件: 2~8℃
规格: 150ml

Sephacryl S-200 HR, 150 ml/2000元 现货 

Sephacryl High Resolution gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

  • Cost-efficient gel filtration media providing high resolution with fast and easy operation.
  • Five different fractionation ranges available in Sephacryl HR family allows for reproducible separations over a wide molecular weight range.
  • Sephacryl S-200 High Resolution medium is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 103 – 2.5 × 105).
  • BioProcess medium supported for industrial applications and well-established in approved processes.

 

Sephacryl High Resolution chromatography media is a highly versatile gel filtration media that offers a wide range of fractionation capabilities. The matrix of Sephacryl High Resolution media is a cross-linked copolymer of allyl dextran and N,N’-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the media minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl HR the medium of choice for routine purification.

 

Sephacryl High Resolution is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.As a BioProcess medium, Sephacryl High Resolution, meets industrial demands with security of supply and comprehensive technical and regulatory support

 

Five different Sephacryl High Resolution chromatography media, with different fractionation ranges, are available:

 

Sephacryl S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 103–1 × 105). Sephacryl S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 103–2.5 × 105). Sephacryl S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 104–1.5 × 106). Sephacryl S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 104–8 × 106). Sephacryl S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 104–2 × 107)

BioProcess MediumYesParticle size d(50v)50 µm1)MatrixAllyl dextran and N, N’-methylenebisacrylamideParticle Size25 µm-75 µmpH Stability Working Range3-11pH Stability Cleaning-in-Place (CIP)2-13Pressure/Flow Specification>150 cm/h, 100 kPa, XK 50/30, bed height 15 cmFractionation [Mr] Globular Proteins5 x 103-2.5 x 105Fractionation [Mr] Dextrans1 x 103-8 x 104Fractionation Range [Mr] DNA Exclusion Limit30 bpExclusion Limit [Mr] [Globular Proteins]≈ 4 x 105 (400 kDa)Storage Conditions4 to 30°C, 20% EthanolChemical StabilityAll commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40°C for 1 week), 0.5 M NaOH (only for cleaning-in-place)
1)d50v is the median particle size of the cumulative volume distribution.
温馨提示:不可用于临床治疗。

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发布于 : 2024-05-17 阅读()
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